Antibody modification is a crucial aspect of antibody engineering, enabling the customization of antibodies for diverse applications in therapeutics, diagnostics, and research. CD ComputaBio's antibody modification service, leveraging computer - aided drug design, offers a comprehensive suite of solutions to modify antibodies with precision and efficiency. Our service aims to enhance the properties of antibodies such as binding affinity, specificity, stability, and pharmacokinetics, while also enabling the addition of new functionalities.
Site - Specific Modification Design
We use various algorithms to identify specific amino acid residues in the antibody that are prime candidates for substitution. Based on the analysis of the antibody's structure and its interaction with the antigen, we can predict which amino acid changes will lead to enhanced binding affinity, altered specificity, or improved stability.
Conjugation and Labeling Design
We design antibody - drug conjugates (ADCs) to improve the therapeutic potential of antibodies. CADD helps in predicting the optimal attachment sites on the antibody for the drug molecule, ensuring that the conjugation does not interfere with the antibody's binding to the antigen or its stability.
Disulfide Bond Engineering
Disulfide bonds play a crucial role in antibody stability. We use CADD to analyze the antibody structure and identify potential sites for the introduction or modification of disulfide bonds. By strategically adding or relocating disulfide bonds, we can increase the antibody's conformational stability.
T - cell Epitope Prediction and Modification
To reduce the immunogenicity of antibodies, we use computational methods to predict T - cell epitopes within the antibody sequence. These are regions that can potentially trigger an immune response. Based on these predictions, we design mutations to eliminate or modify these epitopes while maintaining the antibody's functionality.
Initial Assessment: We start by thoroughly characterizing the client - provided antibody. This includes sequence analysis, structural determination (if available), and measurement of its binding affinity, specificity, and other relevant properties.
Mutational Analysis: Using various software, we perform a comprehensive mutational analysis of the antibody. This involves predicting the effects of various amino acid substitutions on the antibody's structure and function.
Final Product Delivery: We generate a comprehensive report that details the entire process of antibody modification. This includes the initial antibody characterization, the computational design and validation steps, the experimental procedures and results.
At the core of our services lie our cutting-edge algorithms, meticulously developed and continuously optimized by our team of computational biologists and bioinformaticians.
MM/PBSA Algorithm
The MM/PBSA (Molecular Mechanics/Poisson - Boltzmann Surface Area) algorithm is used for calculating the binding free energy between the antibody and antigen. It combines molecular mechanics force fields with a continuum solvation model (Poisson - Boltzmann equation).
Homology Modeling - based Algorithm
This algorithm is based on the principle of homology. When the structure of the antibody of interest is not known, we use homology modeling. We search for homologous antibodies with known structures in databases. We are able to build a model of the antibody's structure.
Multidisciplinary Expertise
Our team consists of experts in computational biology, molecular biology, biochemistry, and immunology. This multidisciplinary expertise allows us to address all aspects of antibody modification, from computational design to experimental validation.
Tailored Service
We offer customized solutions to meet the specific needs of each client. Whether it's a small - scale project or a large - scale industrial project for antibody drug development, we can design a service package that fits the client's requirements in terms of time.
Advanced Resources
We have access to high - performance computing resources that allow us to run complex computational simulations in a timely manner. This is crucial for handling large - scale projects, such as high - throughput virtual screening of modified antibody libraries and molecular dynamics simulations for stability analysis.
CD ComputaBio's antibody modification service offers a comprehensive and innovative solution for customizing antibodies to meet specific requirements. Our service combines the power of computational modeling with experimental validation to provide efficient, accurate, and cost - effective antibody modification. With our four feature services, three approaches, two algorithms, and four advantages, we are well - positioned to meet the diverse needs of clients in the fields of biotechnology, pharmaceuticals, and research.
